---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-17 03:21:00+00:00
layout: post
slug: rna-isolation-hard-clam-gill-hemos
title: RNA Isolation - Hard clam gill, hemos
categories:
  - 2009
  - Miscellaneous
tags:
  - gill
  - Hard clam
  - hemocyte
  - Mercenaria mercenaria
  - NanoDrop1000
  - RNA isolation
  - RNA quantification
---

RNA was isolated from hard clam gill (0.08g; from [20090109](/Sam%27s+Notebook#sjw20090109)) and from hard clam hemos (from [20090114](/Sam%27s+Notebook#sjw20090114)). One "unknown" sample (from 20090114) was also processed. Pellets were resuspended in 50uL of 0.1%DEPC-H2O. Hemo samples were pooled. Samples were spec'd.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090116%20RNA%20SJW.PNG)

Results: 260/280 looks OK for hemos. The gill sample has excellent 260/280 and pretty good 260/230. Samples will be reprecipitated over the weekend @ -20C according to [Ambion PolyA Purist protocol](https://aquacul4.fish.washington.edu/Protocols:Information%20Sheets/Commercial%20Protocols:Manuals/Ambion%20-%20MicroPoly%28A%29Purist%20Kit.pdf) in preparation for mRNA isolation.
