---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-14 22:57:12+00:00
layout: post
slug: rna-precipitation-continued-from-yesterday
title: RNA - Precipitation continued from yesterday
categories:
  - 2009
  - Miscellaneous
tags:
  - EtOH precipitation
  - Hard clam
  - hemocyte
  - hemolymph
  - Mercenaria mercenaria
  - NanoDrop1000
  - RNA
  - RNA quantification
---

Transferred supe to a fresh tube and added 1mL 70% EtOH to remaining pellet. Spun samples max speed @ 4C 30 mins. Removed supe and washed pellets with 1mL 70% EtOH. Spun max speed 10 mins. Removed supe . Resuspended the "supe" sample in 50uL 0.1%DEPC-H2O and the "pellet" sample in 100uL 0.1%DEPC-H2O.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090114%20RNA%20SJW.png)

Results: 260/280 ratios look good. The 260/230 ratios are still horrible. Total yield from these two samples are ~5ug. Will get more hemolymph from clams in order to use more total RNA in the mRNA isolation to maximize cost saving.