---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-13 23:00:35+00:00
layout: post
slug: rna-reprecipitation-of-hard-clam-rna-from-yesterday
title: RNA - Reprecipitation of hard clam RNA from yesterday
categories:
  - 2009
  - Miscellaneous
tags:
  - EtOH precipitation
  - Hard clam
  - hemocyte
  - hemolymph
  - Mercenaria mercenaria
  - RNA
---

Because of the relatively large size of the pellets vs. the amount of RNA, I think another round of precipitation would be best to help remove additional residual salt carryover. Will precipitate O/N according to Ambion PolyA Purist protocol. RNA pellets were resuspended in 250uL of 0.1%DEPC-H2O and precipitated O/N @ -20C.

NOTE: Upon adding 100% EtOH to sample, the solution turned very cloudy and a white precipitate immediately formed inside the tube. I do not think this precipitate is RNA. Tomorrow, before spinning the tube, I will transfer the supe to a fresh tube and process both tubes simultaneously. Hopefully this will remove/eliminate most of the excess salt or whatever seems to be forming the pellet.
