---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-12 23:02:13+00:00
layout: post
slug: rna-isolation-hard-clam-hemolymph-from-20090108-20090109
title: RNA Isolation - Hard Clam hemolymph from 20090108, 20090109
categories:
  - 2009
  - Miscellaneous
tags:
  - Hard clam
  - hemolymph
  - Mercenaria mercenaria
  - NanoDrop1000
  - RNA isolation
  - RNA quantification
  - TriReagent
---

1mL of TriReagent was used to isolate RNA from 3 combined tubes of hemolymph. This resulted in 10 total RNA preps. Pellets were resuspended in 100uL of 0.1% DEPC-H2O and pooled into a single tube and NanoDropped.

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090112%20RNA%20SJW.jpg)

Results: RNA solution looked very cloudy and contains a fair amount of insoluble "stuff". 260/280 ratios also looked bad. Will precipitate O/N according to Ambion PolyA Purist Kit before isolating mRNA tomorrow.
