---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-01-07 23:07:00+00:00
layout: post
slug: pcr-dungan-isolates
title: PCR - Dungan Isolates
categories:
  - 2009
  - Miscellaneous
tags:
  - BC05Ca-18t/H5
  - Chelex
  - DNA Isolation
  - Dungan isolates
  - gel
  - Immomix
  - PCR
  - VATm-1.2t
  - VNTc-1.5t
  - VNTc-12-C1/G10
  - xCvC-11t
  - xCvC-12t
  - xCvC-17t
---

Samples (in Chelex) were vortexed and heated @ 95C for 30mins with periodic vortexing. Tubes were spun max speed @ 4C for 2 mins to pellet Chelex. Set up PCR using Immomix master mix. Anealing temp. = 56C. [PCR set up here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090107-01.jpg).

![](https://eagle.fish.washington.edu/Arabidopsis/20090108.png)

Lane 1 - 100bp ladder

Lane 2 - xCvC-11t

Lane 3 - xCvC-12t

Lane 4 - xCvC-17t

Lane 5 - VNTc-12-C1/G10

Lane 6 - BC05Ca-18t/H5

Lane 7 - VATm-1.2t

Lane 8 - VNTc-1.5t

Lane 9 - Neg. Control

Results: PCR seems to have worked for some of the samples. The bottom-most band in lanes 4, 5, 6, 9, & 10 were cut out and stored in "Sam's Misc. -20C Box". Date is 1/8/2009, since this PCR ran O/N.
