--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2008-12-24 17:43:58+00:00 layout: post slug: rrna-removal-v-tubiashii-total-rna-from-yesterday title: rRNA Removal - V. tubiashii total RNA from yesterday categories: - 2008 - Miscellaneous tags: - MICROBExpress Kit - NanoDrop1000 - RNA - RNA quantification - rRNA - total RNA - Vibrio tubiashii --- rRNA removal was continued from O/N precipitation. Processed the samples according to the [Ambion MICROBExpress Kit protocol](https://aquacul4.fish.washington.edu/Protocols:Information%20Sheets/Commercial%20Protocols:Manuals/Ambion%20-%20MICROBexpress%20bacterial%20mRNA%20kit.pdf) and resuspended final pellets in 25uL of The RNA Storage Solution. Samples were spec'd on the NanoDrop. ![external image 20081224%20RNA%20SJW.PNG](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20081224%20RNA%20SJW.PNG) ## Samples were stored @ -80C in Sam's RNA Box #1.