---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2008-12-22 17:45:44+00:00
layout: post
slug: rna-isolation-v-tubiashii-from-challenge-see-20081216
title: RNA Isolation - V. tubiashii from challenge (see 20081216)
categories:
  - 2008
  - Miscellaneous
tags:
  - MICROBExpress Kit
  - NanoDrop1000
  - RNA isolation
  - RNA quantification
  - TriReagent
  - Vibrio tubiashii
---

Added 10mL TriReagent to 2 x 50mL pellets (5.63 x 10^11 total bacteria; see calcs on [20081219](https://genefish.wikispaces.com/Sam%27s+Notebook#sjw20081219)) from the control samples collected on [20081219](http://genefish.wikispaces.com/Sam%27s+Notebook#sjw20081219). Added 10mL TriReagent to 1 x 50mL pellet (1.835 x 10^12 total bacteria; see calcs on [20081219](http://genefish.wikispaces.com/Sam%27s+Notebook#sjw20081219)) from the V.tubi + oyster samples collected on [20081219](http://genefish.wikispaces.com/Sam%27s+Notebook#sjw20081219). Scaled rest of RNA protocol to match. Resuspended pellets in 100uL 0.1%DEPC-H2O. Samples were NanoDropped.

![external image 20081222%20RNA%20SJW.PNG](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20081222%20RNA%20SJW.PNG)

The V. tubi + gigas sample was eventually diluted to contain 400uL (see final reading for that sample above).

RNA samples were precipitated O/N @ -20C according to Ambion protocol.
