Bismark report for: ../../data/Haws-10/zr3644_4_R1_val_1_val_1_val_1.fq.gz and ../../data/Haws-10/zr3644_4_R2_val_2_val_2_val_2.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-oyster-oa/data/Haws-10/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 49306248 Number of paired-end alignments with a unique best hit: 31176029 Mapping efficiency: 63.2% Sequence pairs with no alignments under any condition: 10173471 Sequence pairs did not map uniquely: 7956748 Sequence pairs which were discarded because genomic sequence could not be extracted: 23 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7791298 ((converted) top strand) GA/CT/CT: 7783361 (complementary to (converted) top strand) GA/CT/GA: 7793769 (complementary to (converted) bottom strand) CT/GA/GA: 7807578 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1095445149 Total methylated C's in CpG context: 14041365 Total methylated C's in CHG context: 897413 Total methylated C's in CHH context: 3904845 Total methylated C's in Unknown context: 59802 Total unmethylated C's in CpG context: 124009766 Total unmethylated C's in CHG context: 186504782 Total unmethylated C's in CHH context: 766086978 Total unmethylated C's in Unknown context: 3665861 C methylated in CpG context: 10.2% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.6% Bismark completed in 0d 4h 20m 36s