Bismark report for: ../../data/Haws-10/zr3644_2_R1_val_1_val_1_val_1.fq.gz and ../../data/Haws-10/zr3644_2_R2_val_2_val_2_val_2.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-oyster-oa/data/Haws-10/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 55025914 Number of paired-end alignments with a unique best hit: 34518538 Mapping efficiency: 62.7% Sequence pairs with no alignments under any condition: 11517458 Sequence pairs did not map uniquely: 8989918 Sequence pairs which were discarded because genomic sequence could not be extracted: 24 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8625829 ((converted) top strand) GA/CT/CT: 8615896 (complementary to (converted) top strand) GA/CT/GA: 8634917 (complementary to (converted) bottom strand) CT/GA/GA: 8641872 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1219646118 Total methylated C's in CpG context: 15827434 Total methylated C's in CHG context: 1026185 Total methylated C's in CHH context: 4563014 Total methylated C's in Unknown context: 62203 Total unmethylated C's in CpG context: 137507065 Total unmethylated C's in CHG context: 208064918 Total unmethylated C's in CHH context: 852657502 Total unmethylated C's in Unknown context: 4058384 C methylated in CpG context: 10.3% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.5% Bismark completed in 0d 4h 47m 45s