Bismark report for: ../../data/Haws-10/zr3644_23_R1_val_1_val_1_val_1.fq.gz and ../../data/Haws-10/zr3644_23_R2_val_2_val_2_val_2.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-oyster-oa/data/Haws-10/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 39902509 Number of paired-end alignments with a unique best hit: 25336717 Mapping efficiency: 63.5% Sequence pairs with no alignments under any condition: 7862442 Sequence pairs did not map uniquely: 6703350 Sequence pairs which were discarded because genomic sequence could not be extracted: 13 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6349154 ((converted) top strand) GA/CT/CT: 6304328 (complementary to (converted) top strand) GA/CT/GA: 6317472 (complementary to (converted) bottom strand) CT/GA/GA: 6365750 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 886020469 Total methylated C's in CpG context: 11770515 Total methylated C's in CHG context: 774415 Total methylated C's in CHH context: 3436488 Total methylated C's in Unknown context: 49699 Total unmethylated C's in CpG context: 101065913 Total unmethylated C's in CHG context: 153146269 Total unmethylated C's in CHH context: 615826869 Total unmethylated C's in Unknown context: 2879731 C methylated in CpG context: 10.4% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.7% Bismark completed in 0d 3h 32m 30s