Bismark report for: ../../data/Haws-10/zr3644_18_R1_val_1_val_1_val_1.fq.gz and ../../data/Haws-10/zr3644_18_R2_val_2_val_2_val_2.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-oyster-oa/data/Haws-10/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 50943423 Number of paired-end alignments with a unique best hit: 32453802 Mapping efficiency: 63.7% Sequence pairs with no alignments under any condition: 10076246 Sequence pairs did not map uniquely: 8413375 Sequence pairs which were discarded because genomic sequence could not be extracted: 17 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8119057 ((converted) top strand) GA/CT/CT: 8092160 (complementary to (converted) top strand) GA/CT/GA: 8110541 (complementary to (converted) bottom strand) CT/GA/GA: 8132027 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1137312620 Total methylated C's in CpG context: 15566929 Total methylated C's in CHG context: 998068 Total methylated C's in CHH context: 4389184 Total methylated C's in Unknown context: 61859 Total unmethylated C's in CpG context: 128925509 Total unmethylated C's in CHG context: 197551089 Total unmethylated C's in CHH context: 789881841 Total unmethylated C's in Unknown context: 3672115 C methylated in CpG context: 10.8% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.7% Bismark completed in 0d 4h 32m 54s