Bismark report for: ../../data/Haws-10/zr3644_14_R1_val_1_val_1_val_1.fq.gz and ../../data/Haws-10/zr3644_14_R2_val_2_val_2_val_2.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-oyster-oa/data/Haws-10/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 36340861 Number of paired-end alignments with a unique best hit: 23105310 Mapping efficiency: 63.6% Sequence pairs with no alignments under any condition: 7331089 Sequence pairs did not map uniquely: 5904462 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5746453 ((converted) top strand) GA/CT/CT: 5794524 (complementary to (converted) top strand) GA/CT/GA: 5807850 (complementary to (converted) bottom strand) CT/GA/GA: 5756482 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 808330026 Total methylated C's in CpG context: 12187417 Total methylated C's in CHG context: 724676 Total methylated C's in CHH context: 3166224 Total methylated C's in Unknown context: 47921 Total unmethylated C's in CpG context: 90151049 Total unmethylated C's in CHG context: 138926513 Total unmethylated C's in CHH context: 563174147 Total unmethylated C's in Unknown context: 2653578 C methylated in CpG context: 11.9% C methylated in CHG context: 0.5% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.8% Bismark completed in 0d 3h 19m 12s