Bismark report for: ../../data/Haws-10/zr3644_13_R1_val_1_val_1_val_1.fq.gz and ../../data/Haws-10/zr3644_13_R2_val_2_val_2_val_2.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-oyster-oa/data/Haws-10/ with the specified options: -q --score-min L,0,-0.8 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53303436 Number of paired-end alignments with a unique best hit: 33654394 Mapping efficiency: 63.1% Sequence pairs with no alignments under any condition: 11108037 Sequence pairs did not map uniquely: 8541005 Sequence pairs which were discarded because genomic sequence could not be extracted: 3 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8404655 ((converted) top strand) GA/CT/CT: 8407185 (complementary to (converted) top strand) GA/CT/GA: 8430117 (complementary to (converted) bottom strand) CT/GA/GA: 8412434 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1183484900 Total methylated C's in CpG context: 15795082 Total methylated C's in CHG context: 1024928 Total methylated C's in CHH context: 4539184 Total methylated C's in Unknown context: 64518 Total unmethylated C's in CpG context: 133362904 Total unmethylated C's in CHG context: 203159538 Total unmethylated C's in CHH context: 825603264 Total unmethylated C's in Unknown context: 3907789 C methylated in CpG context: 10.6% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.6% Bismark completed in 0d 4h 48m 16s