Bismark report for: ../data/69M_R1.fastp-trim.fq.gz and ../data/69M_R2.fastp-trim.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/16TB_HDD_01/sr320/github/project-mytilus-methylation/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 93353347 Number of paired-end alignments with a unique best hit: 55738899 Mapping efficiency: 59.7% Sequence pairs with no alignments under any condition: 18715079 Sequence pairs did not map uniquely: 18899369 Sequence pairs which were discarded because genomic sequence could not be extracted: 107 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 27885435 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 27853357 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2302063801 Total methylated C's in CpG context: 34544232 Total methylated C's in CHG context: 3654003 Total methylated C's in CHH context: 16549176 Total methylated C's in Unknown context: 437157 Total unmethylated C's in CpG context: 253318940 Total unmethylated C's in CHG context: 372392432 Total unmethylated C's in CHH context: 1621605018 Total unmethylated C's in Unknown context: 12747802 C methylated in CpG context: 12.0% C methylated in CHG context: 1.0% C methylated in CHH context: 1.0% C methylated in unknown context (CN or CHN): 3.3% Bismark completed in 0d 6h 22m 33s