Bismark report for: ../data/raw-wgbs/242M_1.fastq.gz and ../data/raw-wgbs/242M_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/sr320/github/project-mytilus-methylation/output/01-bismark-init/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 86793768 Number of paired-end alignments with a unique best hit: 40866671 Mapping efficiency: 47.1% Sequence pairs with no alignments under any condition: 31531236 Sequence pairs did not map uniquely: 14395861 Sequence pairs which were discarded because genomic sequence could not be extracted: 102 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20472681 ((converted) top strand) GA/CT/CT: 8285 (complementary to (converted) top strand) GA/CT/GA: 7939 (complementary to (converted) bottom strand) CT/GA/GA: 20377664 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2099732888 Total methylated C's in CpG context: 39803056 Total methylated C's in CHG context: 10013472 Total methylated C's in CHH context: 48291258 Total methylated C's in Unknown context: 2784447 Total unmethylated C's in CpG context: 240672757 Total unmethylated C's in CHG context: 345973129 Total unmethylated C's in CHH context: 1414979216 Total unmethylated C's in Unknown context: 9128068 C methylated in CpG context: 14.2% C methylated in CHG context: 2.8% C methylated in CHH context: 3.3% C methylated in Unknown context (CN or CHN): 23.4% Bismark completed in 0d 5h 40m 1s