Bismark report for: ../data/raw-wgbs/240M_1.fastq.gz and ../data/raw-wgbs/240M_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/sr320/github/project-mytilus-methylation/output/01-bismark-init/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 88519096 Number of paired-end alignments with a unique best hit: 41790082 Mapping efficiency: 47.2% Sequence pairs with no alignments under any condition: 33591162 Sequence pairs did not map uniquely: 13137852 Sequence pairs which were discarded because genomic sequence could not be extracted: 75 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20924523 ((converted) top strand) GA/CT/CT: 7243 (complementary to (converted) top strand) GA/CT/GA: 7836 (complementary to (converted) bottom strand) CT/GA/GA: 20850405 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2045234260 Total methylated C's in CpG context: 37524903 Total methylated C's in CHG context: 9610115 Total methylated C's in CHH context: 47132319 Total methylated C's in Unknown context: 2490069 Total unmethylated C's in CpG context: 216432074 Total unmethylated C's in CHG context: 328259272 Total unmethylated C's in CHH context: 1406275577 Total unmethylated C's in Unknown context: 9381700 C methylated in CpG context: 14.8% C methylated in CHG context: 2.8% C methylated in CHH context: 3.2% C methylated in Unknown context (CN or CHN): 21.0% Bismark completed in 0d 5h 22m 56s