Bismark report for: ../data/raw-wgbs/239M_1.fastq.gz and ../data/raw-wgbs/239M_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/sr320/github/project-mytilus-methylation/output/01-bismark-init/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 108112211 Number of paired-end alignments with a unique best hit: 50719282 Mapping efficiency: 46.9% Sequence pairs with no alignments under any condition: 44684722 Sequence pairs did not map uniquely: 12708207 Sequence pairs which were discarded because genomic sequence could not be extracted: 71 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 25371954 ((converted) top strand) GA/CT/CT: 5556 (complementary to (converted) top strand) GA/CT/GA: 5051 (complementary to (converted) bottom strand) CT/GA/GA: 25336650 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2378079434 Total methylated C's in CpG context: 39483148 Total methylated C's in CHG context: 11296787 Total methylated C's in CHH context: 55941539 Total methylated C's in Unknown context: 3115796 Total unmethylated C's in CpG context: 232242348 Total unmethylated C's in CHG context: 368776059 Total unmethylated C's in CHH context: 1670339553 Total unmethylated C's in Unknown context: 10902559 C methylated in CpG context: 14.5% C methylated in CHG context: 3.0% C methylated in CHH context: 3.2% C methylated in Unknown context (CN or CHN): 22.2% Bismark completed in 0d 6h 15m 44s