Bismark report for: ../data/raw-wgbs/106M_1.fastq.gz and ../data/raw-wgbs/106M_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/sr320/github/project-mytilus-methylation/output/01-bismark-init/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 88285661 Number of paired-end alignments with a unique best hit: 41908461 Mapping efficiency: 47.5% Sequence pairs with no alignments under any condition: 36332293 Sequence pairs did not map uniquely: 10044907 Sequence pairs which were discarded because genomic sequence could not be extracted: 50 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20973596 ((converted) top strand) GA/CT/CT: 6005 (complementary to (converted) top strand) GA/CT/GA: 4866 (complementary to (converted) bottom strand) CT/GA/GA: 20923944 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1962807409 Total methylated C's in CpG context: 31979766 Total methylated C's in CHG context: 9086543 Total methylated C's in CHH context: 44917378 Total methylated C's in Unknown context: 2178645 Total unmethylated C's in CpG context: 191761640 Total unmethylated C's in CHG context: 305459310 Total unmethylated C's in CHH context: 1379602772 Total unmethylated C's in Unknown context: 8586471 C methylated in CpG context: 14.3% C methylated in CHG context: 2.9% C methylated in CHH context: 3.2% C methylated in Unknown context (CN or CHN): 20.2% Bismark completed in 0d 4h 49m 35s