Bismark report for: ../data/raw-wgbs/105M_1.fastq.gz and ../data/raw-wgbs/105M_2.fastq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/sr320/github/project-mytilus-methylation/output/01-bismark-init/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 92976446 Number of paired-end alignments with a unique best hit: 43928243 Mapping efficiency: 47.2% Sequence pairs with no alignments under any condition: 37402868 Sequence pairs did not map uniquely: 11645335 Sequence pairs which were discarded because genomic sequence could not be extracted: 77 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 21990891 ((converted) top strand) GA/CT/CT: 6461 (complementary to (converted) top strand) GA/CT/GA: 6205 (complementary to (converted) bottom strand) CT/GA/GA: 21924609 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2085981248 Total methylated C's in CpG context: 31378944 Total methylated C's in CHG context: 9663023 Total methylated C's in CHH context: 48051307 Total methylated C's in Unknown context: 2340545 Total unmethylated C's in CpG context: 212943760 Total unmethylated C's in CHG context: 326653235 Total unmethylated C's in CHH context: 1457290979 Total unmethylated C's in Unknown context: 8800113 C methylated in CpG context: 12.8% C methylated in CHG context: 2.9% C methylated in CHH context: 3.2% C methylated in Unknown context (CN or CHN): 21.0% Bismark completed in 0d 5h 19m 27s