FastQCFastQC Report
Fri 22 Mar 2024
RESUB-94.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-94.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15623530
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1138580.7287597617183825No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA288060.18437574607019028No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC163580.10470105027480985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)