FastQCFastQC Report
Fri 22 Mar 2024
RESUB-94.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-94.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15623530
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG237860.1522447231835571TruSeq Adapter, Index 27 (97% over 38bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA215810.13813139540167937No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG205310.13141076312459476TruSeq Adapter, Index 27 (97% over 38bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT186580.11942243526270951No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG175030.11202973975791641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)