FastQCFastQC Report
Fri 22 Mar 2024
RESUB-156.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-156.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15298692
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG236350.15449033159174655TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG223000.14576409538802404TruSeq Adapter, Index 27 (97% over 38bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA160990.10523121845972191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)