FastQCFastQC Report
Fri 22 Mar 2024
RESUB-116.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-116.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14831891
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT239780.16166515786827182No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG235500.1587794840185921TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG233730.15758610955271987TruSeq Adapter, Index 27 (97% over 38bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA222560.15005504018334548No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG202490.13652338734150624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)