FastQCFastQC Report
Fri 22 Mar 2024
98.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename98.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20752442
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6543333.1530409770570618No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA633710.30536647205181927No Hit
GGAGATTTAGCATTTTAGGTCCGCCATCCATCGATAATACACAATGGCCG418140.20148954036349073No Hit
CGGACACGTACATATTCAACACGCATGTGCTTTATGTGCTATGAACAACT396050.19084500995111806No Hit
AGGAGATTTAGCATTTTAGGTCCGCCATCCATCGATAATACACAATGGCC297550.1433807163513576No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA280990.13540093257458566No Hit
CCTCACTTCATGCATGCATGCGGAAACTCATGCAAGCAAAGACATACACG261790.12614900935513998No Hit
TTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTCA254110.12244824006736171No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221590.10677779511442556No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA209110.10076404502178586No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC208130.10029181144079333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)