FastQCFastQC Report
Fri 22 Mar 2024
97.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename97.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16826686
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2490331.4799883946250616No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA547000.32507886579686573No Hit
TGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAA343850.20434802194561663No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA302300.17965510261497716No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA255200.1516638510993787No Hit
GGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAAGAA243210.14453826499169237No Hit
GTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAAT235750.14010483109983748No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233400.13870823999449444No Hit
TGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATA201590.11980374507493632No Hit
GTGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAA198310.1178544604683299No Hit
CCTCACTTCATGCATGCATGCGGAAACTCATGCAAGCAAAGACATACACG195240.11602997762007326No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC185540.11026532497248716No Hit
GGAGATTTAGCATTTTAGGTCCGCCATCCATCGATAATACACAATGGCCG180220.1071036804276255No Hit
GTTGGGTTGACTTGGTGATGTTAATAACAAAAGATACATACCAAACCTAT177830.10568331755878727No Hit
CGGACACGTACATATTCAACACGCATGTGCTTTATGTGCTATGAACAACT175080.10404900881849224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)