FastQCFastQC Report
Fri 22 Mar 2024
97.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename97.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16826686
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGGGG623930.37079790993900996TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT578170.3436030125005007No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGGGG472010.28051275218423877TruSeq Adapter, Index 10 (97% over 37bp)
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC416760.24767800385649322No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGTGG390760.2322263575846129TruSeq Adapter, Index 10 (97% over 37bp)
CAAAAATGAAGCCATAGCAGTAATCAATAATACCTTCTAGAGTCTGACTA348170.20691537240309826No Hit
CTTTATTCAAATAGATTAAATGAAAATACACTGTTACAATCCAGATTTTT325920.19369232895889305No Hit
GGAAAATCTGTTATACCAAAAATGAAGCCATAGCAGTAATCAATAATACC285380.1695996466565074No Hit
TTTTGCATCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACT268840.15977002245124203No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245580.145946741978783No Hit
AAGAAATCGAAAGATTCGAAACACAAATCAAATTCATATTTGGATTGAAA230960.13725816242128722No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGTGG227000.1349047578352624TruSeq Adapter, Index 10 (97% over 37bp)
CACCAAGTCAACCCAACGAAATTAACCGGATACAATTTAATTTATACCAC222610.13229580679166414No Hit
TGCATCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTGTT218600.1299126875012703No Hit
TCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTGTTACAA208040.12363694193853739No Hit
CGGAAAATCTGTTATACCAAAAATGAAGCCATAGCAGTAATCAATAATAC195340.11608940702881125No Hit
TTTGCATCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTG188320.11191746253540358No Hit
CATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTGTTACAAT184770.10980771852520455No Hit
TTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGCAGTAA178080.10583189108063229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)