FastQCFastQC Report
Fri 22 Mar 2024
91.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename91.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18832038
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGGGG402300.21362531235334167TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT335520.17816446632063931No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGGGG258660.1373510397546989TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGTGG252050.13384106382962904TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG241990.1284991034958617No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA231400.12287570787612045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)