FastQCFastQC Report
Fri 22 Mar 2024
90.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename90.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17003449
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3282281.9303613049328994No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA734550.4320005899979469No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC267490.15731514235729468No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254730.14981078250653734No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA209440.12317500996415491No Hit
GGGACTCAATAGCTTTCGATCTCACACACACTCACACACACACACACACA203840.11988156049987271No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA195400.11491786166441878No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA186310.10957188744471784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)