FastQCFastQC Report
Fri 22 Mar 2024
90.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename90.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17003449
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG945890.5562930203160548TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT730430.42957755217779636No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG663980.39049724558823334TruSeq Adapter, Index 10 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308420.1813867292453431No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC269910.1587383830186452No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC267870.15753862642808528No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG220870.12989717556714525No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG218620.1285739146216747TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG194900.11462380367653646TruSeq Adapter, Index 10 (97% over 37bp)
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC179580.10561386692782151No Hit
TGAGTATCCACATACAAGTCCAACACAACACATAATAAGTTGTTCATAGC175510.10322023490645926No Hit
TTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACCTT173470.10202047831589932No Hit
TGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCA171560.10089717680218878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)