FastQCFastQC Report
Fri 22 Mar 2024
88.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18736055
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4258192.2727249679828545No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA927950.49527501920761874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314270.16773541708753523No Hit
GGGACTCAATAGCTTTCGATCTCACACACACTCACACACACACACACACA245400.13097741226741702No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC227680.12151971159350247No Hit
CACACACTCACACACACACACACACACACACACACACACACACACACACA208980.1115389552389764No Hit
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT201050.10730647406831373No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA199960.106724708056205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)