FastQCFastQC Report
Fri 22 Mar 2024
88.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18736055
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG1169540.6242189190840868TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG939580.501482302437733TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT833750.44499762623455147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474800.25341513995342135No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC462040.24660474149974473No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC352460.18811857672279464No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG297890.158992914997314TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG293540.15667118825174242TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG252250.1346334647288343No Hit
TGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCA241510.12890120145356104No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC209670.11190722913654982No Hit
TTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACCTT196770.10502210844278584No Hit
GTGAGTATCCACATACAAGTCCAACACAACACATAATAAGTTGTTCATAG196740.1050060965341957No Hit
TGAGTATCCACATACAAGTCCAACACAACACATAATAAGTTGTTCATAGC194790.10396532247583604No Hit
TGAATATGTACGTGTCCGTGTATGTCTTTGCTTGCATGAGTTTCCGCATG192410.10269504439435088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)