FastQCFastQC Report
Fri 22 Mar 2024
83.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename83.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15437259
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1266790.8206055232991816No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA371570.2406968750087046No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT260000.16842368195027368No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC232010.15029222480493462No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA195380.12656391915170953No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA192650.12479547049123163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)