FastQCFastQC Report
Fri 22 Mar 2024
78.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename78.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16961387
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2954011.7416087493316437No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA531220.3131937264328678No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC281450.16593572211989502No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT202470.11937113397624852No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA193080.11383503011870431No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA181090.10676603275427889No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA178520.10525082648016934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)