FastQCFastQC Report
Fri 22 Mar 2024
78.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename78.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16961387
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG835280.4924597263183724TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT665720.3924914866926862No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG614520.36230527609564006TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG226630.13361525210172964No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC213840.12607459519672537No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG198470.11701283627335429TruSeq Adapter, Index 10 (97% over 37bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA194940.11493163855055015No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG184090.10853475603144955TruSeq Adapter, Index 10 (97% over 37bp)
GCACGATGTTCTTCTGCATAGGCGGCGTTGATTTGTTGCTGTTTTTGTAA181580.10705492422288343No Hit
GTTGATTTGTTGCTGTTTTTGTAACTAGACACACATTCTCAGCAGAAAAA178950.10550434348323048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)