FastQCFastQC Report
Fri 22 Mar 2024
69.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename69.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16372600
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG384900.23508789074429232TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG322440.19693878797503148TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT248990.15207725101694294No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC189510.11574826234074002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)