FastQCFastQC Report
Fri 22 Mar 2024
58-S.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename58-S.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14552346
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG1405310.9656930916843236TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG1255740.8629124128851801TruSeq Adapter, Index 27 (97% over 38bp)
CTCAGATGCAACTGTAAAACCTTGGGGTGCTTATTTTTAAAACTAGCCAA343620.23612687603771929No Hit
GTTTTTATTGACCATTGAAACGGACCACATGCATCAATGATGGCGGGATG185810.12768387997371694No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180650.12413805993892668No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATGGGGG170690.11729380266247105TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATAGGGG148630.10213473483931731TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)