FastQCFastQC Report
Fri 22 Mar 2024
57-S.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename57-S.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12171985
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG4096263.365317982235436TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG3602312.959509069391722TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATGGGGG488080.400986363358154TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATAGGGG424820.34901456089536753TruSeq Adapter, Index 27 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286200.23513009587179082No Hit
CTCAGATGCAACTGTAAAACCTTGGGGTGCTTATTTTTAAAACTAGCCAA268060.2202270213116431No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGGGGGG218330.1793709078675335TruSeq Adapter, Index 27 (97% over 38bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA216380.17776886843025194No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCGAGGGG182900.15026308363015564TruSeq Adapter, Index 27 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181600.14919505733863456No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGT170580.14014148062127912TruSeq Adapter, Index 27 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG167120.1372988875684615No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGT154240.12671721169554515TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)