FastQCFastQC Report
Fri 22 Mar 2024
5.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18540171
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2119191.1430261349800928No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA640860.34566024229226366No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA311520.16802434022857718No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC277430.14963723905243376No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT233360.12586723175314835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)