FastQCFastQC Report
Fri 22 Mar 2024
5.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18540171
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTGGGGG1147810.6190935347899434TruSeq Adapter, Index 9 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT673280.36314659665220994No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG259460.13994477181467205No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC246640.13303005673464394No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA216050.11653074828705734No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC210480.11352646100189691No Hit
TGAGTATCCACATACAAGTCCAACACAACACATAATAAGTTGTTCATAGC188820.10184372085888528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)