FastQCFastQC Report
Fri 22 Mar 2024
48.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename48.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18761830
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5545082.9555112694230785No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA329260.17549460793536664No Hit
TTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTCA313020.16683873588024195No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC298020.15884378016430167No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA250490.13351043048572556No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236430.12601649199465084No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA214430.11429055694460509No Hit
TGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAA210220.11204663937366452No Hit
CTTGAGTCCCAGGAGAACTTTGAGTCTTTCATGAAGGCAATTGGTCTCCC209130.11146567259163953No Hit
CTTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTC201620.10746286476319207No Hit
GGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAAGAA199220.10618367184864162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)