FastQCFastQC Report
Fri 22 Mar 2024
48.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename48.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18761830
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG4073782.171312713098882TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT337110.17967863476004206No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321370.17128926122878207No Hit
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC294690.15706889999536292No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG256320.13661780327398765TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG223900.11933803898660206TruSeq Adapter, Index 2 (97% over 37bp)
CAAAAATGAAGCCATAGCAGTAATCAATAATACCTTCTAGAGTCTGACTA212590.11330984237678308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)