FastQCFastQC Report
Fri 22 Mar 2024
41.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15181149
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12323438.117587147059817No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGTTTAAGCCATGCATGTATAAG238050.15680631288185104No Hit
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT213860.14087207760097736No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAACTAATGTG185010.1218682459410681No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA180570.1189435661292831No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180550.11893039189589667No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC165310.10889162605544546No Hit
CAAAGTTTAAGCCATGCATGTATAAGTCCACACGGCTGGAAAAGTGAAAC155210.10223863819530392No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)