FastQCFastQC Report
Fri 22 Mar 2024
41.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15181149
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG9733386.411490987935103TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG563980.37150020726362676TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG547860.3608817751541731TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGGGGGG373020.24571262689009904TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGT252210.16613367011943564TruSeq Adapter, Index 2 (97% over 37bp)
CTTTTACTTCCTCTAGATAGTCAAGTTTGATCGTCTTCTCGGCACGCGCG240430.1583740466548349No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217940.14355962121180682No Hit
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT181280.11941125141450097No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC166490.10966890582524418No Hit

[WARN]Adapter Content

Adapter graph