FastQCFastQC Report
Fri 22 Mar 2024
40.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename40.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15520211
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8002965.156476287596863No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA578710.3728750852678485No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298630.19241362118079453No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC220790.14225966386668323No Hit
TTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTCA170220.10967634396207629No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA158300.10199603600750017No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)