FastQCFastQC Report
Fri 22 Mar 2024
40.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename40.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15520211
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG6119413.9428652097577794TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT393270.2533921735986708No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377370.24314746751832172No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG369710.2382119676079146TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG341250.21987458804522694TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGGGGGG253740.16349004533507955TruSeq Adapter, Index 2 (97% over 37bp)
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC231120.1489155012132245No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC168760.10873563510186814No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGT161300.10392899941888677TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACCTT157840.10169964828442087No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)