FastQCFastQC Report
Fri 22 Mar 2024
38.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename38.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036199
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6474683.8005425975594678No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC286050.16790717225127508No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA282400.16576467555937802No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190800.1119968133736874No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA181520.10654958890771352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)