FastQCFastQC Report
Fri 22 Mar 2024
38.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename38.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036199
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG5020952.947224319227546TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT338340.19860063855793184No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG301300.17685869952563948TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG267760.15717120937598814TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241950.14202111632999825No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC228980.13440791575632569No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGGGGGG206350.12112443626656393TruSeq Adapter, Index 2 (97% over 37bp)
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC194340.11407474167213004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)