FastQCFastQC Report
Fri 22 Mar 2024
37.flexbar_trim.R_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename37.flexbar_trim.R_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15243144
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG884920.5805364037760189TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT210880.1383441631201542No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180300.1182826849893959No Hit
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC159100.10437479302170208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)