FastQCFastQC Report
Fri 22 Mar 2024
31.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename31.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17724988
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1307400.7376027560639251No Hit
TCCAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAG1169140.6595998823807384No Hit
CCAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGA421860.23800298200483974No Hit
TGAAGACTTCAGCGGTTGTTCTGGTGCTTGCGTTGGCCTTCGGCTTTGGA240160.13549233432485258No Hit
CAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGAT225050.12696764590193232No Hit
CAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGATGAAGAC192490.1085980989098554No Hit
GAAGACTTCAGCGGTTGTTCTGGTGCTTGCGTTGGCCTTCGGCTTTGGAG192260.10846833859633642No Hit
CTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGATGAAGACTTCAGCGGT181180.10221727653637903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)