FastQCFastQC Report
Fri 22 Mar 2024
3.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19136269
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1984491.0370307817056712No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA617530.3227013583473351No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA298610.15604400209884173No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT201230.10515633951424909No Hit
CACACATACACACACACACACACACACACACACACACACACACACACACA195200.10200525504736582No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG194470.10162378047674811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)